Confocal images are represented as the average projection of a stack of sections (Z-series) or as a single section.
Contacts can also be illustrated as they appear throughout the stack of sections as a simple 0.5 μm layer or as a transparency of all layers merged together.
Images can be illustrated as they appear throughout the stack of sections as a simple 0.5 μm layer or as a transparency of all layers merged together.
We followed the reviewer's suggestion and imaged again all used SNARE markers and integrated the signals through a stack of sections covering the entire cell.
The new set of data and its quantification is now shown in Figure 6 figure supplement 3. Reviewer #1: 1) Integrate the fluorescence signals of the various SNARE proteins through a stack of sections covering the entire cell.
The authors could rule out that their results are artefacts by addressing several points: 1) Integrate the fluorescence signals of the various SNARE proteins through a stack of sections covering the entire cell.
In fact, a double disector (Gundersen 1986; Lucocq 1992, 1994) from a single stack of sections was used to estimate both the packing density of cells in a reference volume (Ncell/ Vref, where ref represents the centrifuge pellet) and the packing density of gold particles in the same reference volume (N g / Vref).
This enabled defined stacks of sections to be visualized as well as the 3-dimensional stack through the entire retina to be rotated 90°.
(C) Representative image of a 3D-rendered model of a confocal image stack of a section from tumor sample 45 hybridized with HER2 (red) and CEP17 (green) probes.
By rotating the entire stack of optical sections 90° (Fig 1, A3 E3), we created a cross-sectional view where the cones are on the top and the inner retina at the bottom.
MacLeod and his colleagues create their designs on a computer, which renders every adornment or edible sculpture as a stack of cross sections, each thinner than a sheet of paper.
