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Data acquisition in hyperspectral SRS imaging can be performed by either wavelength scanning over a stack of frames, lines, or at each pixel.
Spectral scanning over a stack of frames is subjected to long-term fluctuation and can result in spectral and spatial distortions.
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Stacks of frame-averaged (four frames) confocal optical slices were collected digitally, at 5 minute intervals for time-lapse sequences.
(c) Z-projection stack of 30 frames from our processed dataset registered with the ZBB atlas.
(a) Z-projection stack of 30 frames from our processed dataset registered with the ZBB atlas.
(b) Z-projection stack of 26 frames from stimulus cluster 3 correlated pixels registered with the ZBB atlas for the same area of interest as (a).
(e) Z-projection stack of 28 frames from stimulus cluster 3 correlated pixels registered with the ZBB atlas for the same area of interest as (d).
Working on one of the "Police Story" films with Jackie Chan, she had to run through a stack of bed frames, several of which collapsed on her head, sending her to the hospital for 17 stitches.
The stack of image frames was degraded by both shot (Poisson) noise and read out (Gaussian) noise.
Within minutes, both the owner and his assistant had suitably oohed and ahhed over the photo --someone even mentioned how good my hair looked -- and then competently assembled a stack of six frame samples to try.
In the present implementation, with its 400-Hz frame rate, a stack of 29 adjacent frames covers a time interval of 72.5 ms, resulting in 3,915-D 3,915-D vectors.
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