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Partial fluorescence yield data were recorded in scanning mode by a grating with line density 1200 lines mm−1 and radius 7.5 m dispersing the emitted photon energy from the sample,[ 14] and subsequently a detector consisting of a charge-coupled device, fluorescence screen and microchannel plate stack collecting the amplified signal.
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Hosein Mortada, a Lebanese journalist working in Syria for Press TV, recorded this video: My colleague Liam Stack collected other videos for Watching Syria's War.
Figure 7 shows the thickness of nanofiber stacks collected with an array of 2, 4 and 8 nozzles equidistantly spaced at 0.5 cm apart in the single tube and double tube cases.
Spindle pole or total kinetochore intensity was measured using Imaris software (Bitplane Scientific Software) from Z-stacks collected as previously described.
Between 600 and 800 rod cell nuclei were scored in stacks collected from each retina area.
[ 17], with z stacks collected every 30 s for 20 min.
Maximum intensity projections of Z-stacks collected every 1 min for a time period of 60 minutes.
Dendritic segments to be analyzed were selected and identified in image stacks collected either 2 hr or 24 hr apart.
Co-localization between GFP and microglial markers (Iba1, CD68-positive cells) was analyzed on sequential confocal images (Z-stacks) collected at 0.25-μm intervals covering 3-μm depth (12 total scanning images for each channel).
For z-stacking analysis, stacks were collected at 1 μm intervals, and a single image was generated by deep focus post-processing.
For convenience of derivation, we stack the collected data in (3) into a M r L × 1 vector as (59).
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