Sentence examples for stably expression from inspiring English sources

To generate the U2OS-Cas9 celineine, the cells were treated with 1.5 μg/ml puromycin to select the U2OS cells stably expression of Cas9.

A total of 5 μg/ml blasticidin was used to select the U2OS PARP2- or APLF-null cells stably expression of Cas9.

Measurement of telomere repeat fragments (TRF) in the breast cancer cell lines stably expression TGF-β family cytokine receptor mutants showed consistent results.

These results suggest not only that the inhibitory effect of GD3 is not specific for a particular hepatoma cell line, but also that exogenous GD3 antagonizes the c-Src/NF-kB pathway induce by hypoxia, reproducing the effects observed with the stably expression of GD3 synthase in Hep3B-GD3 cells, which minimizes the potential contribution of artifacts during clone selection.

Long term of stably expression of C/EBPα in prostate cancer cells stimulates cell proliferation.

This may explain the difference of cell fates between transient and stably expression of C/EBPα.

In our previous study, the utility of N. lugens 18 s rRNA gene has been validated for their stably expressions in N. lugens tissues, developmental stage and immune-induced individuals [ 2, 12, 13].

In further support of Nrf2 regulating a STING-dependent antiviral response we observed that in HEK293 cells stably expressing STING the expression of Nrf2 reduced the IFN-response to known STING agonists, including HSV-1 (Supplementary Fig. 5b d).

As an additional control, cell extracts from cells stably expressing the empty expression vector were used.

Cells were virally transduced with the constitutive MYCN expression constructs, stably expressing cells were selected, and total cellular RNA was harvested.

Normalization of input cDNA was performed by quantifying gene expression of stably expressed internal reference genes as described earlier (Ceelen et al., 2014; Messiaen et al., 2012).