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Therefore, we used RNA interference to knock down TIP5 expression and constructed stably transgenic shRNA-expressing NIH/3T3 or miRNA-expressing HEK293T and CHO-K1 using shRNA/miRNA sequences specific for two different regions of TIP5 (TIP5-1 and TIP5-2).
SOX4 expression in CaSki cells was upregulated by stably transfecting a constructed SOX4-expressing plasmid.
We infected HCC cells with a lentivirus to deliver shRNA targeting ASNS and constructed stably transfected MHCCLM3 and MHCC97H cells.
For this, we constructed stably transfected MCF-7 cells (MCF-7-COX-2) and mock control cells (MCF-7-mock) as previously described.
Stably transfected cells were constructed as described previously [ 3]. Cell lines were stably transfected with cDNA encoding for CCK2R or with empty vector ('mock-transfected').
L. lactis strain sAGX0085 was constructed by stably inserting an htff1 expression cassette into the bacterial genome, and clinically formulated as a mouth rinse (coded AG013).
The system designed and constructed can stably generate the gas stream with the PCDD/F concentration ranging from 1.0 to 100 ng TEQ N m−3 while reproducibility test indicates that the PCDD/F recovery efficiencies are between 93% and 112%.
Fluorescent gene expression reporters for different phases of the YMC were constructed and stably integrated into the yeast genome.
The lentiviral expression vector pLVX-IRES-ZsGreen-miR-124 (Clontech Laboratories) was constructed to stably overexpressing mature sequence of miR-124 in MDA-231-D3H2LN.
A cell line stably expressing pGFPint was constructed and subjected to knockdown of NMD factors UPF1, UPF2 and SMG1 or transfection with a control siRNA, C2.
A modified ICAD gene was constructed and stably transduced into TK6 and L929 cells as described previously (Yan et al, 2006a).
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