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We then used two elements to mask different sites in this mRNA: antisense LNA oligonucleotides to mask nucleotides by stably base pairing to them and EJCs to mask nucleotides in a sequence independent manner.
This showed that miRNA-mRNAs chimeras recovered are strongly enriched for stably base-paired interactions.
Third applet uses raw Cq data and determines the most stably expressed genes based on coefficient of correlation (r) to the BestKeeper Index (BI).
BestKeeper determines the most stably expressed genes based on the coefficient of correlation to the BestKeeper Index, which is the geometric mean of the candidate reference gene Cq values.
Of the 136 stably transformed lines based on PCR detection of the faeB gene and Southern blot (Additional file 2), 18 showed detectable levels of faeB activity using a microplate rapid screening assay [ 17].
Expression of Wnt luciferase reporter was normalized to cell number based on stably integrated SV40-driven Renilla luciferase for HEK293 cells or on the MultiTox-Fluor cell viability assay (AFC fluorescence; Promega) for Hs578T cells.
The other laboratories used reporter gene assays, two based on stably transfected human prostate carcinoma cells (assay 2) or human mammary carcinoma cells (assay 4), and the third based on transient transfection of Chinese hamster ovary cells (assay 3).
Alternatively, protein binding molecules can be selected from protein libraries based on small, stably folded protein scaffolds presenting flexible loops, which are randomized in the libraries.
These analyses are relatively conventional approaches to exploiting the information after the earthquake based on the stably recorded triggered data.
CFB boiler burned semi-coke from pyrolyzer stably, and also could run based on the requirements of the respective adjustment of pyrolyzer and boiler load.
(2) CFB boiler burned semi-coke from pyrolyzer stably, and also could run based on the requirements of the respective adjustment of pyrolyzer and boiler load.
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