Sentence examples for stable transposition from inspiring English sources

This system allows for single, stable transposition events since transposase activity is lost along with the plasmid.

Indeed, stable transposition of fluorophores into the genome has been employed to study the progeny of GFAP, GLAST and nestin-expressing lineages in the developing murine cortex (García-Marqués and López-Mascaraque, 2013; Siddiqi et al., 2014).

We could transfect these cells, and even if the efficiency was low, this proved sufficient to study the subcellular localization of fusion proteins, obtain stable transfectants using transposition-mediated integration and subclone the transformed cells.

Additional developments for plant-gene tagging systems with anther culture could replace the promoter with a mid- to late-uninucleate microspore specific one so that the transposition is stable after integration.

The results demonstrate that transient or constitutive sources of SB11 transposase effectively mobilize transposition from a stable T2/OncZ concatemer followed by genome-wide re-integration.

For example P450 SU1 has been used in Ac/ Ds transposon-mediated mutagenesis screens to select for progeny in which the Ac transposase gene had segregated away from the Ds element, thereby ensuring that the location of the Ds element was stable after the initial Ac-mediated transposition event [ 21].

Altogether, we obtained stable transfectant of Speedy cells using transposition-mediated integration and subcloned the transformed cells.

The piggyBac transposon system has recently attracted a great deal of attention because the system is a nonviral gene-delivery approach that uses DNA-transposition ability for the stable expression of exogenous genes via a 'cut-and-paste' mechanism.

In addition, if the nucleotide composition of the chimeric element provides a stable, secondary structure, it could acquire a transposition advantage.

Certainly, the genome of the final stable plasmid-less multi-integrant strain may contain transposition-mediated chromosomal rearrangements, including inversions and/or deletions of bacterial DNA fragments (Watson et al. 2004).

The rate of transposition is given by the number of stable transfectants obtained after drug selection.