Sentence examples for stable transformation of from inspiring English sources

While stable transformation of gametes or regeneration of whole plants from transformed somatic cells is routinely achieved in model plant species (e.g., by floral dipping in Arabidopsis thaliana), for most non-model species, the lack of suitable protocols and the phenomenon of regeneration recalcitrance make the obtainment of whole-plant transformants an arduous task.

The plasmids allow either transient expression of Kaede-containing protein constructs in plant cells or Gateway cloning and stable transformation of plants.

The analysis based on transient and stable transformation of tobacco showed that the artificially designed multifactorial activation module activated transcription simultaneously to comparable levels in both the directions.

To study the role of CKX2 in relation to rice productivity, we specifically down-regulated the expression of OsCKX2 by RNAi in this study, using stable transformation of short hairpin RNAs (shRNAs) that comprised the N-glycosylation site sequences of OsCKX2 (Brummelkamp et al. 2002).

Stable transformation of cv.

Stable transformation of bloodstream forms and procyclic forms were performed as described [16], [34].

Stable transformations of rice were performed as described above for transient expression assays with three exceptions: three-month-old rice callus cultures were used and 150 ng of the pHLucGWgus vectors (containing the PvUbi1, PvUbi1+3, PvUbi1+9, PvUbi2, PvUbi2+3, PvUbi2+9, ZmUbi1, and CaMV 35S promoters) were used per bombardment.

Stable transformation rescue of gna-2 qa705 gna-2 qa705trated wash a fragment of the cosmidemonstratedleotides 20340–23478, Genbank accession number Z81130) sub-cloned into pBSKS- to yield the construct pwith

One of the important advantages of RNAi type of silent lines obtained in the process of stable transformation is inheritance of the traits.

Compared to the DVS-I (pLA-1 and pHf1g3T-1), the DVS-II (pLT-2 and pHf1g3A-2) stable stransformationmation efficiency regardless of the order of the vectors introduced into the host cells.

The efficiency of stable transformation, calculated as the percentage of explants (petioles) producing stable transgenic plants, varied with the construct.