For C2C12, growth medium consisted of Dulbecco's modified Eagle's medium (DMEM) including 4.5 g/L glucose and stable glutamine, supplemented with 10% fetal calf serum (FCS).
They were maintained in high-glucose DMEM with stable glutamine supplemented with 10% foetal calf serum, 100 units per ml penicillin G sodium and 100 μg ml−1 streptomycin sulphate (PAA, Cölbe, Germany).
Human epithelial ovarian cancer cell lines SKOV3, OVCAR3, OVCAR5, A1847, A2780, and C30 were acquired from ATCC and cultured in RPMI 1640 with stable glutamine, supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Biosera).
The results clearly stress the importance of adding a stable N-resource supplement as the key factor to the stability and high efficiency of the AD system.> The groups with the supplement maintained a significantly higher methane production during the whole process.
Stable transfectants were supplemented with Geneticine® G-418.
Supplementing stable fixations with external fixator not only increases surgical time but also subjects the patients to increased risks of complications of added surgery.
RTCs were grown in complete liquid phase medium (RPMI 1640 with stable L-glutamine) supplemented with 10% heat-inactivated (30 min at 56°C) fetal bovine serum, 100 IU/mL penicillin, and 100 μg/mL streptomycin (Sigma).
A first conclusion of this study is thus related to the general belief that LasR and other LuxR family members require the presence of appropriate acyl-HSL molecules to properly fold into their active conformations, which is mostly based on the production of large amounts of soluble and stable protein upon supplementing the bacterial growth medium with cognate acyl-HSLs.
A20 (BALB/c, H-2d, B cell lymphoma) cells, a gift of Prof. K. Smith, were maintained in RPMI-1640 medium with stable glutamine and supplemented with penicillin/streptomycin (all PAA), 10% fetal bovine serum (Life Technologies), and 50 μM β-mercaptoethanol (Sigma).
A20 cells (BALB/c B cell lymphoma, H-2d), a gift of Professor K. Smith, and YAC-1 cells, were maintained in RPMI-1640 medium with stable Glutamine and supplemented with penicillin/streptomycin (all PAA), 10% FBS (Life technologies), and 50 μM β-mercaptoethanol (Sigma).
