To achieve 'physiologically relevant' tissues in vitro additional complexity must be introduced to provide suitable transport, cell signaling, and matrix support for cells in 3D environments to achieve stable readouts of tissue function.
Besides high sensitivity, the Raman scatter possesses 10~100 times narrower bands than those of fluorescence and excellent anti-photobleaching properties, which avail to reduce undesirable spectral overlap and provide long and stable signal readout [9].
The probe was inserted into the rectum up to 2 cm deep and held in the same position for 15 seconds until a stable temperature readout was obtained.
This paper presents a technique for the compensation of this capacitive crosstalk, enabling stable closed-loop readout interfaces.
DsRed provides a stable and cumulative readout of endogenous hsp70 induction: Whole-mount antibody labelling with hsp70 (green) (A-D) and DsRed (E,F) in non-heat shocked control (A,C,E) and heat shocked (B,D,F) zebrafish embryos at 29 hpf (A,B), 39 hpf (C,D) and 48 hpf (E,F).
The combinatorial nature of a histone code has led to the hypothesis that dynamic histone phosphorylation at S10 and S28 might affect the readout of stable methylation marks at the respective neighboring residues K9 and K27 [39].
To enable assessment of biological readouts, we also performed stable transduction of FLT3 ITD expressing 32D cells with Dusp6-specific shRNA.
Operation of the two outermost layers and the z-coordinate readout in all layers is stable.
In order to achieve high-throughput screening of HIV PIs in a relevant setting with a high readout, we have produced a stable T-cell line that can be used as a sensitive indicator of HIV-1 PR activity.
Although it could be argued that activation of NF-κB may have occurred more rapidly than the earliest time point examined in our study (4 hours), this appears unlikely because the firefly luciferase protein used as a readout in these experiments is stable for up to 6 hours in mammalian cells [ 34].
