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The data used in the analysis were stable readings made during the approach.
Each pressure and temperature level was kept constant for 5 min to get stable readings of sample resistance.
This again means that stable readings can be obtained if the treated sample has an area response corresponding to that level.
For each 15 min or 30 min measurement, the mean value was calculated from 33% of the values (sampling frequency every 10 sec) which represented the most stable readings in the cycle.
Practically, we found that an acclimation period was necessary to obtain stable readings.
Seven-minute in-vivo vessel diameter recordings performed on 9 Brown-Norway rats showed stable readings.
Similar(42)
In vitro assessment of PCL and PCL/PAL composites in tissue culture medium in the absence of cells revealed stable pH readings with time.
Clear pumping for 10 min was carried out until stable meter readings of the in-situ parameters (temperature, pH, Eh and EC) was obtained using portable digital meters.
We also noted that use of TEpacVV in time-lapse FLIM measurements yielded much more stable FRET readings than use of CEpacVV (Fig. 1E).
Pilot testing showed stable measurement readings between 0 and +35 degrees centigrade.
After mounting in the QCM-D system (QCM-D D4, Q-Sense AB, Sweden) and acquisition of the resonance frequencies, the sensors were always equilibrated for typically 30 min to ensure stable frequency readings in the respective buffer (ethanol for thiol SAM formation, aqueous 12.5 mM NaCl for protein adsorption experiments).
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