Sentence examples for stable overhang from inspiring English sources

Under the stable overhang is a useful place to dry rugs, giving the benefits of being outside and also having some protection if it rains, however makes sure that all the horses will be fine with this and won't spook too badly.

If necessary hang them inside or under the stables overhang.

Finally, LIC uses larger overhangs (10 30 bp) than Golden Gate based assembly; these overhangs remain stable during transformation and eliminate the need for a prior ligation step.

To generate 16-mer overhangs at a stable and low rate, it is necessary to optimize reaction temperature and time.

Ligation-independent cloning (LIC) [ 27] employs longer overhangs resulting in sufficiently stable DNA base pairing for transformation.

For a given coding sequence, the tabulated Δ G for an off-target complex is the most stable predicted of the 3 possible overhangs for that coding sequence.

Interestingly, even if these miRNA-like are well represented and predicted to originate from stable structures, we identified 2 nucleotide 3′ overhangs at only one strand of the putative dsRNAs.

Furthermore, we discarded all sequences where mfold predicted off-target heteroduplex (e.g., the structure of Figure 2 C where and strand form a stable duplex that displays only single nucleotide overhangs) or homoduplex Δ G within 5.5 kcal/mol of the desired heteroduplex Δ G.

The pri-miRNA is processed by the RNase Drosha in the nucleus into ∼70 nucleotide precursor miRNA (pre-miRNA) products, which locally fold into stable secondary hairpin loops with ∼2 nucleotide 3′-overhangs [22].

For stable knockdown of Wnt11 and Kaiso, shRNA sequences flanked by a BbsI and XhoI overhang (Wnt11 5′-TCCCGTCTGCAAGTGAGACC -ATATTCAAGAGATATGGTCTCACTTGCAGACTTTTTC-3′; Kaiso 5′-TCCCTCAGAAGATCATTACATTAGATTTCAAGAGAATCTAATGTAA -TGATCTTCTGATTTTTC-3′) were andealigated lintoed into pH1tetflex (Herold et al., 2008).

The continuous stacking of nts in the transition region between stem II and the U6 3′-overhang seen in our structure may properly orient these elements for stable Prp3 binding.