Weight change was categorized as weight loss (loss of ≥2 kg), stable (loss or gain of ≤2 kg), or weight gain (gain of ≥2 kg).
A stable loss of function allele was generated with the TALEN genome editing system targeting an mwoI restriction enzyme site located within exon seven of gba1.
Recently, powerful genome-editing techniques have been developed to generate stable loss-of-function models for neurodegenerative diseases (Schmid and Haass, 2013).
The stable loss of expression of CD26, a T-cell activation marker, in MF/SS suggests that this activation pathway may be nonfunctional in most MF/SS tumors.
At −18 °C, concentrations were stable (loss <15%%) for 84 days, both with and without NaF, and they were unstable at +4 °C, with a loss of 25 and 50 % by day 14, with and without NaF, respectively.
Stable loss of methylation in HCT116 cells does result in a minor increase of several thousand binding sites, largely corresponding to selective reactivation of binding sites from other cell types, especially from other malignant cell lines.
In 22Rv1 cells, similar to DU145 cells, stable loss of SPRY2 significantly enhanced cell growth as well as upregulated EGFR/HER2 phosphorylation along with PI3K/AKT activation as their downstream signalling partners (Supporting Information Fig S13).
Surprisingly, stable loss of BAP1 had minimal or even paradoxical effects in most standard assays of tumorigenicity, including cell cycle control, motility and the ability to form colonies in soft agar, suggesting that BAP1 loss promotes tumor progression in a manner that is different from most characterized tumor suppressors.
In order to evaluate the stability of I-XCI in undifferentiated TS cells, we used a female TS cell line (F3) carrying a mutation at the X-linked Hprt locus (Hprt bm1 ) associated with a retroviral insertion in the maternal Hprt1 gene, which leads to a stable loss-of-function of the maternal allele [ 28].
