Most proteins are only barely stable, which impedes research, complicates therapeutic applications, and makes proteins susceptible to pathologically destabilizing mutations.
Additional blocks at the level of reverse transcription and integration may further impede stable transduction of naïve cells with non-viral sequences, accounting for a nearly eight orders of magnitude difference between the replication efficiencies of constructs with or without specific retroviral cis-elements[10].
During scanning, the GTP bound to eIF2 in the TC is hydrolyzed in the 43S PIC in a manner dependent on the GTPase activating protein eIF5, but Pi release is blocked by eIF1, which also impedes stable binding of Met-tRNAi in the P site.
The concentration of GelMA was chosen from a range which is above a lower critical concentration of hydrogel formation (4.5 wt %) and below a concentration that impedes stable droplet formation within microchannels due to its high viscosity (15 wt %; Figure S3 in Supporting Information).
To make the transition smoother we have changed the sentence as below: "During scanning, the GTP bound to eIF2 in the TC is hydrolyzed in the 43S PIC in a manner dependent on the GTPase activating protein eIF5, but Pi release is blocked by eIF1, which also impedes stable binding of Met-tRNAi in the P site".
Specifically, there are two main types of atherosclerotic lesions: the stable plaque which simply impedes blood flows and an unstable one which is vulnerable to rupture.
Thus, DNA hypomethylation in Tert−/−S ESCs arose in response to critically short telomeres and impeded their stable differentiation.
However, such hepatocyte cultures are phenotypically and functionally not very stable over time which impedes their use for long-term toxicity testing (Berthiaume et al. 1996).
Being undocumented has always been a precarious way to live, but the increased targeting of migrants under Trump has further impeded building a stable life.
The extremely high G+C content of the 5' half of the deleted segment (Figure 1b) creates the potential for the RNA to form stable secondary structures, which could impede the procession of reverse transcriptase (RT) and DNA polymerases.
