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The pattern of pGT47A expression in Physcomitrella was analysed by histochemical staining of 4 independent stable pGT47A-GUS lines, each of which showed a similar staining pattern.
After transformation, two stable Ppα-DOX-GUS lines, Ppα-DOX-GUS-12 and Ppα-DOX-GUS-2, expressing the corresponding fusion proteins from the native Ppα-DOX promoter were selected for further studies (Additional file 3).
The stable expression of GUS driven by PvUbi1 and PvUbi2 promoters in rice leaves, stems and roots also demonstrates the ubiquitous nature of these promoters in mature plants.
All of the other bacterial treatments such as cyanide mutants PAO6344 and CHAO77, showed stable expression of DR5::GUS similar to the control untreated plants.
Thus, the system (2.1) is GUS (globally uniformly stable).
The GUS-1 is stable to heat upon calcination at 700 °C in air.
To further examine the cell type and tissue specific expression of the ACR11 gene, we fused the putative promoter of ACR11 to a β-glucuronidase reporter gene (ACR11p-GUS) and generated stable Arabidopsis transgenic lines.
The FABP7 relative mRNA expression level was normalized with respect to the beta-glucuronidase (GUS) gene, which had stable transcript levels under these experimental conditions.
GUS staining was performed on stable transformants as previously described [12].
MAb 323/A3 and GUS were linked through a stable thioether bond.
NLS-4, a line important for RNAi based loss-of-function studies, is a stable transgenic line that expresses a GFP-GUS fusion with a nuclear localization signal [11], [13], [38].
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