Sentence examples for stable expressed from inspiring English sources

Therefore the needed normalization factor is calculated out of the expression data of co-amplified genes that are stable expressed in the certain sample material, the so-called reference genes.

To get more reliable quantification results, we performed an experiment in advance to screen reference genes for qPCR (see Methods for details), and the relative expression levels of unigenes were normalized to 3 stable expressed reference genes.

Also in mouse expression arrays genes encoding for ribosomal proteins are the most stable expressed ones.

One reference gene would be enough to show a rough expression mode of genes, if the reference gene was identified as a stable expressed gene (Cho et al. 2014).

In the current study, among the 5 traditional reference genes B-TUB11, B-TUB2, Rps-24, UBC, and α-ACTIN) tested, UBC was identified as a stable expressed gene (Cho et al. 2014); however, multiple reference genes have been reported in other studies (Kong et al. 2015).

None of the commonly used housekeeping genes were present in the top 50 of the most stable expressed genes.

Most of the regenerated lines now showed gene expression levels very similar to those of the stable expressing lines (PNA 8.6.1 and ICE 1.1.1).

Stable expressing cells were generated by transfection with 2 μg p3xFLAG-CMV14-Occ expression vectors using FuGENE® HD (Roche).

The chapter describes the features and the design of the retroviral plasmids and describes the way in which they are used for the generation of stable expressing cells.

Stable expressing Sp2/O-Ag14 cells were established after co-transfection with the two pcDNA3 plasmids encoding cTA12 light and heavy chains using the limiting dilution cloning method.

To generate the stable expressing human APP695 in HEK cells, we transfected with pcDNA-APP695, selected by immunoblotting and maintained positive clones in the presence of 200 mg/ml G418 (Gibco- Invitrogen, Carlsbad, CA).