Sentence examples for stable express from inspiring English sources

48 hours later, puromycin was added at 5 ug/mL to select the CMT93 cells which stable express GFP.

Stable cell line p-miR-181a, which could stable express miR-181a, was established.

The cells resistant G418 and stable express exogenous IκBαM were used for subsequent analyses.

pTARGET-miR-181a vector, which can stable express of miR-181a, contains pri-miR-181a and some of its flanking sequences, and the sequences were amplified by the following primers: 5′-CGCCTCGAGCCCAATATATGTTAATCTCTTACC-3′ (forward) and 5′-GCGCGCGTCGACTTTTTAATAAATTTTTACTTGCTA-3′ (reverse).

Therefore the needed normalization factor is calculated out of the expression data of co-amplified genes that are stable expressed in the certain sample material, the so-called reference genes.

Most of the regenerated lines now showed gene expression levels very similar to those of the stable expressing lines (PNA 8.6.1 and ICE 1.1.1).

To get more reliable quantification results, we performed an experiment in advance to screen reference genes for qPCR (see Methods for details), and the relative expression levels of unigenes were normalized to 3 stable expressed reference genes.

Also in mouse expression arrays genes encoding for ribosomal proteins are the most stable expressed ones.

Stable expressing cells were generated by transfection with 2 μg p3xFLAG-CMV14-Occ expression vectors using FuGENE® HD (Roche).

One reference gene would be enough to show a rough expression mode of genes, if the reference gene was identified as a stable expressed gene (Cho et al. 2014).

The chapter describes the features and the design of the retroviral plasmids and describes the way in which they are used for the generation of stable expressing cells.