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Results revealed that even minor environmental pH change could significantly influence the metabolic activity of the cultured primary cancer cells, demonstrating the importance of stable culture condition for a precise cell-based assay.
It has previously been reported that laminin-511 supports the stable culture of hESCs and hiPSCs11.
Supplementing the culture medium with 0.02% or more carboxymethyl cellulose (CMC), allowed stable culture under conditions that had previously damaged the cells.
The biosensor output was stable, culture samples could be stored 10 days under −20 °C, and this sensing system was resistant to interferences by toxic aromatic pollutants.
Non-contact methods, such as calcium imaging and voltage-sensitive dye proteins, could have an advantage compared with electrode array and patch clamp techniques given that these latter techniques typically require a stable culture environment.
The features of this cell culture platform include maintaining homogenous and stable culture environments, efficient medium delivery and cells/agarose (scaffold) loading; allowing realization of more precise and high-throughput cell culture-based assays.
Similar(29)
Here we report the picoeukaryotic diversity in Blanes Bay (NW Mediterranean) by retrieving environmental 18S rDNA sequences and by obtaining stable cultures.
In consequence, the utilisation of light energy by stable cultures of phytoplankton cells in a steady state in a continuous flow chemostat varies inversely with the rate of cell growth, DPS.
And just like that, a sudden crisis leveled many stable cultures bringing them to their knees through two simple emotions -- fear and anxiety.
Stable cultures were generated and down-regulation was evaluated with RT-qPCR and western blot analyses.
Stable cultures could be maintained over multiple passages in these conditions.
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