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In conclusion, the new plasmid is a convenient tool for constructing stable conditional mutant strains in Mycobacteria and can be used for future target identification.
This study successfully constructed a new plasmid pMDX for generating stable conditional mutant strains in M. tuberculosis using lineated double-stranded DNA electro transformed cells.
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We have addressed this issue by comparing the transcription profiles of stable and conditional EBNA-1 transfected sublines of the EBV negative B-cell lymphoma BJAB where EBNA-1 was expressed for few days, few months or several years.
By using stable and conditional cell lines that express high and low levels of MYCN, we found that inhibition of MYCN expression correlated to marked increase of CDKL5 expression.
Going from small cutting thicknesses to the large ones, the behaviour of the finite amplitude instability can be divided into an unconditional stable region, a conditional stable region, a periodic region and a breakdown region.
In theory, alternative mating tactics may represent either distinct genetic alternatives, maintained in evolutionarily stable proportions, or conditional phenotype expression dependent on the environmentally determined state of the male.
If the set did include an accidental truth P and not the accidental truth Q, then ~P ∨ ~Q would be consistent with the set and so for the set to be stable, the counterfactual conditional (~P ∨ ~Q) → P would have to be true.
Here, we constructed high-producing and stable cells by the conditional expression of mutant cell division cycle 25 homolog B (CDC25B) using the Cre-loxP system.
Whilst we have not analysed outreach with respect to local-level service capacity, we recognize that sustainable outreach is conditional on stable primary health care [ 26].
In the current, more stable environment, Bangladesh's conditional cash-transfer based model to boost women's education may serve as a useful model for Nepalese policymakers and development agencies.
Once an alternative reproductive tactic invades a population, either a mixed evolutionary stable strategy or a conditional strategy in which the alternative reproductive tactic is physiologically or environmentally determined maintains the polymorphism [ 5].
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