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Functional homogeneity or evenness of a microbial population contributes towards the development of a stable colony, and therefore, we determined the functional homogeneity of biofilm-harvested cells.
All these results demonstrated the occurrence of physiological as well as structural adaptations of AKS2 cells in biofilm on LDPE surface that resulted in better attachment, better utilization of polymer and better growth of AKS2 cells, leading to the development of a stable colony on LDPE surface.
Normally you would want to introduce just a few males and a much larger group of females if you were to set up a stable colony.
Heterozygous mutant mice were intercrossed to establish a stable colony of homozygous mice.
AP-2α has been previously shown to activate p21Waf1 leading to inhibition of cellular DNA synthesis and stable colony formation in colon carcinoma cells [17].
HD and TN carried out cell transfection and stable colony selection.
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Stable colonies were picked after 7 9 days.
As shown in figure 2B, in the presence of bFGF, stable iPS colonies emerged after 9 days of doxycycline-induced reprogramming, whereas in the presence of LIF, stable colonies were noted 4 days earlier.
Thus, it appears that while the culture growth factor conditions affect the dynamic of the iPS reprogramming process, with stable colonies emerging late under FGF growth factor conditions, the general outcome of the reprogramming response is not affected by the culture conditions.
Stable colonies are selected by G418 selection (Sigma).
Individual neomycin stable colonies were picked and examined for EGFP fluorescence using confocal microscopy.
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