The instable polyelectrolyte multilayer, hybrid templates, and assembly cycles were demonstrated to be three indispensable factors responsible for the formation of this type of 3D stable capsules.
Our results with iPEMs are in agreement with this theory, as we observed stable capsules from pH 4 9, but collapsed capsules at pH 11 as uncompensated charge on polyIC increased due to decreasing cationic charge on SIIN* at this very basic pH.
This cavitand 2 self-assembled into thermally stable molecular capsules G@22 in the presence of suitable guests through 8 intermolecular hydrogen bonds of imide NH⋯OC, two for each four paired hydantoinyl units.
These data confirm that stable iPEM capsules can be assembled from peptide antigens and adjuvants at different length scales and with control over the composition of the capsules.
In comparison to our recent work with PEMs assembled on gold nanoparticles, here we present a platform for simplifying vaccine design and evaluation by electrostatically assembling stable vaccine capsules solely from immune signals.
Only if larger and stable numbers of capsule colonoscopies should ever be performed, and regarded as economically viable, might such a service appear more realistic.
Exploiting hydrogen bonding interactions, multilayered films were prepared by the alternate deposition of PMAAlk,SH (hydrogen bonding donor) and poly N-vinylpyrrolidone) (poly N-vinylpyrrolidone accePVPON, yielding stable PMAAlk,Shydrogenes.
Incubation of iPEM capsules in PBS, media, or media supplemented with 10% FBS confirmed capsules were stable for at least 96 h.
One can observe that in the acid medium the complexed capsules are much stable than the chitosan beads.
Differential scanning calorimetry (DSC) analysis indicated that the SMEDDSs were semisolids at temperatures up to 50 °C and physically stable and compatible with HPMC capsules for 3 months storage at 25 °C and 4 °C.
