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The achievable stable amplification factor is 10 100.
The amplifier provides an overall gain of 3 × 107 in 61 round trips at an electrical pump energy of 300 J. Stable amplification has been simulated and demonstrated.
Such in vitro system allows a stable amplification and recombinant production of antibody fragments bypassing some of the issues associated to the hybridoma technology.
The AMsw assay shows stable amplification efficiency and a high correlation of the standard curve for the different subtypes tested.
Nine loci which were polymorphic and with stable amplification were used in the present study (Table 3).
Of the reference genes, hsa-miR93-5p was shown to have the most stable amplification in all of the individual samples with good expression (CT values ≤30).
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The gradient dilution PCR assays for these three reference genes showed stable, high amplification efficiencies (Additional file 12).
We first screened for reproducible and stable random amplification polymorphic DNA (RAPD) markers using 47 primers, as described by Debener and Mattiesch [ 21] and by Yamagishi et al. [ 34].
In the analysis, 14 primer combinations produced stable and reproducible amplification patterns in three repetitive experiments.
The optimized reaction system could be repeated stably; and the stable and reliable amplification results were able to clearly distinguish different potato varieties.
The primer pairs designed on the gene open reading frames yield stable and repeatable amplification profiles that show a level of resolution that allows the clear discriminate between different strains.
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