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For other bivalent domain genes, exemplified here by Pax6, Jmjd3 recruitment correlates with transcriptional up-regulation but apparently its H3-K27 demethylactivitytivity sets in only later, likely to enable stable activation in NS cells.
That is if we look at the average PSTH activity of the networks across trials they tend to be either stable activation cells, or stable inhibition cells.
The feedback signal is propagated back to the previous layer once the top level columns start to give a stable activation for all variations of the same pattern.
To reach a desired firing scheme over multiple exposures also known as "stable activation", the error occurring at the top layer generates a feedback signal forcing the column firing for the original pattern to fire and also inhibiting the column that is firing for the new variation.
Additionally, transiently transfected AICD-GV16, corresponding to the γ-secretase mediated APPGV16 cleavage product, produced stable activation of the Gal4-reporter when treated with thapsigargin, tunicamycin or BFA (Figure S2).
In the context of DV boundary formation, the following observation suggests that a new property might have to be implemented to provide stability to the regulatory network: since Wg refines the width of the Notch activation stripe through inhibition of the Notch receptor by Dishevelled, stable activation of Notch at the boundary might require Wg to not negatively act on boundary cells.
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A more alkaline growth medium, for example, made the autoinducer less stable, slowing activation of the cell-suicide gene and increasing the population.
Thus, Cut activity makes boundary cells not only refractory to Serrate and Delta expression [9] but also to any negative input coming from Wg, thus allowing stable Notch activation [10].
The aggressive, invasive phenotype of RASF appears early in RA as a consequence of stable cell activation.
Because of the cross regulatory interactions between dpp and Sog, this leads to a stable BMP activation pattern and expression of the cardinal genes in restricted domains.
MTS reagents were applied to the cytoplasmic face of inside-out patches after stable current activation with PKA and ATP, and the current was monitored for at least 5 min to ensure stable modification.
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