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In the study reported here, no stable burst-phase intermediate could be detected, even in the presence of stabilizing salt (0.4 M Na2SO4).
In the atomic model of Cucumber mosaic virus (CMV), six amino acid residues form stabilizing salt bridges between subunits of the asymmetric unit at the quasi-threefold axis of symmetry.
Among others we observed the destruction of the regular helical twist, the loss of a stabilizing salt bridge and the loss of a stabilizing hydrophobic interaction in the β1 region.
We also generated S475E/C479T, and S475D/C479T Hchannelsnels, in an attempt to introduce a stabilizing salt bridge that has been previously characterized for inward rectifiers [25], [26].
Due to the eminent loss of glutamic acid, the total amount of charged residues for stabilizing salt bridges is reduced.
Finally, these two prototypes also interact with each other, forming a stabilizing salt bridge between Lys-304 and Glu-376.
Similar(48)
As might be expected, many of the least abundant compounds are hormones or signaling molecules while the most abundant molecules serve as buffering agents or stabilizing salts.
In particular, Zn2+ seems to stabilize salt bridges across two SAM domain interfaces by directly binding one amino acid in each pair (Baron et al., 2006).
The basic nitrogen atom of the ligand forms a hydrogen bond stabilized salt bridge with Asp1143.32, the distance between the heavy atoms is 2.7 Å, and the N-H-O=(Asp1143.32) angle is 171°.
Simulations and experiments identified two conformations: an open-like conformation with a small contact interface stabilized by salt bridges, and a closed-like conformation with a larger contact interface stabilized by surface-exposed hydrophobic residues.
Thus, the different modes in which the N-terminus can interact with the intracellular region of the transporter, and variations in the manner in which E428 can stabilize different salt bridges and thereby modulate the mode of Na+/Na2 release, can result in different kinetic pathways even for the relatively simple step of Na+/Na2 release in the transport cycle of hDAT.
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