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The crystals were stabilized and cryoprotected by being transferred first to a stabilization solution containing 100 mM sodium cacodylate buffer (pH 6.5), 20% (w/v) PEG350 MME, and 100 mM CaCl2.
At the end of the experiment, the leading shoot from three plantlets of each of the three lines tested were sampled and pooled in Kennedy Space Center Fixation Tubes KFTT) containing a RNA stabilization solution.
Total RNA was extracted from tissues preserved in RNA stabilization solution with Trizol (Invitrogen, Carlsbad, CA).
Absorbent sponges were kept in remaining stabilization solution in the event that additional extractions were required.
Tissues were preserved in RNA stabilization solution at −20°C for subsequent analysis.
After sample collection, DNA was preserved by placing the sponges in Oragene·ANIMAL stabilization solution, labeled, and then sent to our laboratory by mail.
Similar(20)
Despite the 18 million Euros spent since 1999 on geotechnical investigations and stabilization solutions, the numerous reactivations that occurred through 2010 and 2013 demonstrate the persistent activity of the landslide.
This paper tries to explain the above claim in the aspect of the electrotherapy and control theory to suggest the control signal of electrotherapy based on an assumption for chaos model of patient and its control signals design according to multiple stabilization solutions.
As Lifvendahl stressed, most of the current stabilization solutions are more about image stabilization than video stabilization.
I'd recommend picking up a cheap, portable tripod or monopod, or looking into DIY stabilization solutions.
Together, this represents a comprehensive evaluation using standardized panels of reagents and real-world shipping conditions, of sample stabilization solutions commercially available today that can still enable diagnostic testing for all relevant biological markers.
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