On the basis of observed stabilization interactions it was suggested that channel assembly is initiated by the assembly of the core complex between VirB7, VirB9 and VirB10 across the cell envelope.
They likely reflect the potential for SOC stabilization via interaction with soil mineral surfaces.
Based on this model we observed that Lys219 and Glu227 are positioned to make ionic contacts that would facilitate the stabilization of interaction network involving residues 186, 187, 189 and 190 (Figure 1B).
Additionally, we have presented evidence that multimerization of SpoIIE is required for both stabilization and interaction with the pole.
Recently it was proved that the RecQl5 helicase promotes genome stabilization through interaction with RNA polymerase II [ 40].
In the hypoxic state, hydroxylation of HIF-1a is inhibited, causing stabilization, enabling interaction with HIF-1b and increased transcription of its downstream targets.
Thus, it is possible that the alternative cloverleaf model has a low intrinsic thermodynamic stability and can only be formed in vivo upon stabilization through interactions with other cellular or viral ligands or that the 5'- SL and/or the 3'- SL, but not the complete 4-helical junction, exist transiently in the folding intermediates to ensure the correct folding of RNA11.
The Q564H BARD1 mutation attenuates BARD1-dependent induction of apoptosis, p53 stabilization, and interactions with p53, suggesting that the BARD1 linker region is critical for a functional interaction of BARD1 with p53.
This is the prerequisite for the second step, an intermolecular stabilization through interactions between neighbouring charged segments, leading to the delocalization of the excess charges over several segments and, therefore, to further stabilization of the system.
In our motif, in contrast to the conventional HTH (helix-turn-helix), it is the N terminal helix (α4) which has the role of DNA recognition helix, while the C terminal helix (α5) would rather contribute to the motif stabilization by interactions with the α4 helix.
