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The technical performance includes enzyme stability, substrate specificity, enantioselectivity, and reactivity (Mateo et al. 2007; Schoffelen and van Hest 2012).
Similarly, factors that influence biotransducer performance such as enzyme stability, substrate interference, mediator selection and calibration are reviewed.
Engineering methods have been used to alter the stability, substrate specificity and stereospecificity of aldolases to produce excellent enzymes for biocatalytic processes.
In addition, several other residues/regions have been implicated in protein stability, substrate binding and/or catalytic activity [27], [29], [35], [36].
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In order to explain the stereoselectivity of SADH catalyzed reductions, a model is proposed that emphasizes the importance of the stability of substrate conformation and the steric interaction between substrate, enzyme and coenzyme.
Impacts on enzyme stability and substrate specificity were negligible.
Protein engineering of PLD contributes to the improvement of their activity, stability, and substrate specificity.
Enzymes that exhibit superior catalytic activity, stability and substrate specificity are highly desirable for industrial applications.
Recombination has generated proteins with diverse thermostability and mechanical stability, enzyme substrate specificity, and optogenetic properties.
FDH variants with improved stability, expanded substrate scope, and altered regioselectivity have been developed.
Few examples are cited in this review to highlight the multidisciplinary approaches for designing novel enzyme variants with improved thermo stability and substrate specificity.
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