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These genes were selected from the microarray results for their stability of expression across all samples (< 1.12 fold differentially expressed).
Engineering the nuclear genome of plants is perceived to be associated with problems regarding biosafety and the stability of expression of the transgene.
Moreover, in comparison with other major cereals like rice and maize, the development of a high throughput wheat transformation systems has been slowed and severely affected by genotype effects on plant regeneration, low transformation efficiencies and problems with transgene inheritance and stability of expression.
This contradicts to the results of evaluation of gene expression stability (see above) that demonstrate the stability of expression of SAND and CACS in different buckwheat tissues.
The stability of expression of 18sRNA was validated and confirmed in initial pre-analysis of 32 pairs of control and tumor samples by comparison with ACTB expression (Figure S1).
But isolating recombinant mammalian cell lines for large-scale production remains costly and time-consuming, due to substantial variation and unpredictable stability of expression amongst transfected cells, requiring extensive clone screening to identify suitable high producers.
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Because BestKeeper software is designed to determine a reliable normalization factor but not to compute the goodness of each RG independently, we took as the stability-of-expression value the coefficient of determination of each gene to the BestKeeper index.
A selected number of potential references genes can then be validated experimentally and the stability of expressions analyzed by the above mentioned statistical algorithms in defined experimental settings.
To test the hypothesis that some ribosomal protein genes can serve as reference genes for neuronal differentiation, a genome-wide analysis was performed and putative reference genes were identified based on stability of expressions.
We carefully selected five reference genes for accurate quantification of gene expression by RT-qPCR and checked the stability of their expression.
The expression of CD25, which is associated with the stability of Foxp3 expression [ 22], was not significantly different between C57BL/6 and RORγt Tg mice.
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