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Of these 17 SSRs, five SSRs were polymorphic in repeat number, four SSRs contained SNP polymorphisms in one or more repeats, and five SSRs did not have any polymorphisms detected in the sequence capture reads.
Twenty-six (Table 2) of the 40 SSRs were polymorphic within the three mapping populations and were run on the progenies to determine the linkage groups according to the rose ICM24 and used as quality control markers.
Twenty-six oft of 40 tested anchor SSRs were polymorphic in all three populations and thus used as quality control markers (Table 2; Supplementary Table 1), among which, six SSR markers failed to fit in the final maps though they were initially grouped into the expected LGs along with the SNP markers.
281 of the 842 polymorphic SSRs were polymorphic exclusively in wild species.
In addition, 71 (44.4%) of these SSRs were polymorphic across a panel of 40 individuals.
About 20% (14 SSR) of the tested Nothofagus SSRs were polymorphic and showed at least one individual that differed in allelic composition.
Similar(46)
Microsatellites, or Simple Sequence Repeats (SSRs), are polymorphic loci present in nuclear DNA that consist of repeating units of 1 6 base pairs in length.
For instance, of the 609 putative SSRs that were identified based on bioinformatic screening of EST databases, only 61 are polymorphic in cultivated tomato [ 13] and only 10 to 25 of these SSRs are polymorphic within a given cross (Francis, unpublished).
Following genotyping tests, 11 EST-SSRs were polymorphic and produced reliable electrophoretic profiles in E. gomphocephala (Tables 1 and 2).
Twenty one EST-SSRs were polymorphic with average PIC value of 0.33 and transferability rate of 71%.
Between 21% (VrnA, WSC) and 69% (Xtg-ART) of the EST-SSRs were polymorphic in each mapping population.
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