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We also sequenced wc-1, wc-2, vvd and frq in select accessions with either a large or small number of SSRs in order to identify potential single nucleotide polymorphisms (SNP).
It is also advisable to arrange and test the selected SSRs in order, from the top of chromosome 1 to the bottom of chromosome 10.
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One strategy to better exploit a database of EST-SSRs in order to find polymorphic markers is to first sample the longest SSRs (≥ 30 bp), favoring dinucleotide repeats, then follow with trinucleotide, tetranucleotide and pentanucleotide repeats [ 23].
We also genotyped the Set 2 RILs using an additional 153 SSR markers in order to assess the structure of the whole population.
To achieve a higher success rate, another set of primers was also were designed closer to the SSR motifs in order to capture short length reads included in contigs.
The presence of SSRs was analyzed in order to search for new putative markers for physical and genetic mapping.
We initiated a genome-wide association study using GBS-derived SNPs in order to identify the chromosomal regions associated with SSR resistance.
In order to make SSRs markers readily available for downstream analysis, we integrated only those MISA SSRs markers, which have the corresponding primer pair information.
> -wrap-foot> sleepleep self-report, CSHQ children's sleep habits questionnaire, NS not significant aSpearman's correlation between mean SSR and CSHQ item scores In order to compare sleep in Dutch children to sleep in children from different cultural backgrounds, the results from this study were compared with results found by Liu et al., who used the CSHQ in China and the USA [ 17].
In order to identify SSRs for all possible combinations of dinucleotide, trinucleotide, tetranucleotide and pentanucleotide repeats the SSR webserver (GDR) was run (http://www.rosaceae.org/bio/content title=&url=/cgi-bin/gdr/gdr_ssr).org/bio/content title=&url=/cgi-bin/gdr/gdr_ssr
In order to identify SSRs for all possible combinations of dinucleotide, trinucleotide, tetranucleotide and pentanucleotide repeats, we performed a run using the SSR webserver (GDR) (http://www.rosaceae.org/bio/content title=&url=/cgi-bin/gdr/gdr_ssr).org/bio/content title=&url=/cgi-bin/gdr/gdr_ssr
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