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One thousand three hundred and seventy-two microsatellite markers developed in the authors' lab, including 494 EST-SSRs, 632 GSS-SSRs and 200 SSRs derived from BAC end sequences, in addition to 46 SSR markers developed by Li et al (2001) [7] were used.
The information of SSRs derived from all unigenes is shown in Additional file 11.
In addition to SNPs, SSRs derived from BACs were identified, tested for polymorphisms and mapped.
Here we report the identification and characterization of SSRs derived from peanut BAC end sequences (BES).
According to the original sequences used for identification of simple repeats, SSRs are divided in two categories, genomic SSRs derived from random genomic sequences and EST-SSRs derived from expressed sequence tags.
SSRs derived from compound repeats had an average polymorphism rate of 36.3%, similar to Class I SSRs.
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Finally, the oil palm EST-SSRs derived from vegetative and reproductive development will be useful for studies on the evolution of the functional diversity within the palm family.
We have generated 121 and 257 EST-SSRs derived from leaf tissue of red raspberry (R. idaeus) and black raspberry (R. occidentalis) respectively.
An interesting feature of the present map is that six e-SSRs derived from vitellogenin genes were gathered in only LG11 of the consensus map.
In the present study, transferability of EST-SSRs derived from E. sagittatum is high (85.7%) across 52 species in genus Epimedium.
A total of 52 Epimedium species including 55 individuals (Additional file 3) were selected and investigated for the transferability of EST-SSRs derived from E. sagittatum.
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