Information on repeat motif, repeat number, and SSR start and end positions within the respective ESTs were extracted from the SSR-IT output.
Similarly, SSR-IT was used to screen for perfect repeat motifs (http://www.gramene.org/db/markers/ssrtool[ 47]), and Primer3 (http://frodo.wi.mit.edu) [ 71] was used for primer design.
Unigenes in the transcript assembly were screened for perfect repeat motifs using SSR-IT (; [ 24]) and imperfect repeat motifs using FastPCR.
Unigenes in the transcript assembly were screened for perfect repeat motifs using SSR-IT http://www.gramene.org/db/markers/ssrtool) and for imperfect motifs using FastPCR (http://primerdigital.com/fastpcr.html).html
Although there were several thousand chromosome-specific SSRs it was important to identify the most polymorphic ones for map construction and gene mapping.
Whenever the length of an insertion or a deletion event is not a multiple of 3 (as it would be for tri- and hexa-SSRs), it generates a null allele for the gene (or in the worst case, a dominant negative allele).
Staff-student ratio (SSR) SSRs compare the number of staff teaching a subject with the number of students studying it, to get a ratio where a low SSR is treated positively in the league tables.
Even though Multiple Gateway System [ 5- 8] clone a promoter inside the specific SSR sites, it bears low efficient in subsequent multiple recombination reactions and it is also difficult to insert a cis-element next to the promoter.
Unlike conventional HVDC, the VSC HVDC does not cause Subsynchronous Resonance (SSR) when it is close to turbo-generators.
By contrast, the region is not conserved as such in T. brucei, where it sizes 8286 bp, bears several 'unlikely' CDSs and retroelements and, more importantly, does not actually constitute a SSR; still, it is flanked downstream by the same DGC as in the other Tri-Tryps and upstream by the upstream DGC of L. major chromosome 1 but entirely inverted [ 5].
Surprisingly indeed, the putative transcription initiation site (TIS) previously identified on this chromosome by run-on analysis [ 14] is found in the most polymorphic portion of the SSR; yet, it presents a conserved poly(C) tract in a highly conserved position in divergent Leishmania species.
