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One sequence contained three SSRs, while ten SSRs were of the compound type (SSR containing stretches of two or more different repeats).
Six SSRs were of compound types (SSR containing stretches of two or more different repeats).
The individual sequences within each SSR containing contig was subsequently analysed for SSRs using MISA to identify allelic and/or genotypic SSR motif polymorphisms.
We have developed and screened a microsatellite-enriched small-insert genomic library for identification of A. euteiches SSR containing sequences.
In general, one SSR containing BES was selected from each cluster for designing the primer pairs.
Ninety six primer pairs could be designed form 83.5% of SSR containing unigenes.
Similar(39)
Primers were successfully designed for 68 SSR-containing sequences (62.0%).
The estimated frequency of SSRs was approximately 1/2.8 kb, which could be extrapolated to 1 SSR-containing unigene in 4.6 unigenes.
A cluster analysis was employed to obtain non-redundant SSR-containing sequences, leading to the definition of 576 non-redundant sequences containing 783 SSRs.
Gene ontology (GO) annotations showed that six GO terms were significantly overrepresented within SSR-containing contigs.
Primers were designed from 7,705 of the SSR-containing sequences.
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