Sentence examples for ssp I from inspiring English sources

In vitro transcription was performed after digesting the amplified product with Ssp I. DsrA was obtained by runoff transcription of pBAU10301 that was digested by Ssp I [38].

The plasmid was prepared for runoff transcription by digesting with Ssp I.

DNA for pronuclear injection was purified over a CsCl gradient and digested with Ssp I and EclHK I.

About 400 ng of genomic DNA, isolated from hfr1-23 plants, was digested by Dra I, EcoR V, Pvu II, Sca I, and Ssp I restriction enzymes and linked to an adapter duplex to produce five genomic-walk libraries.

For in vitro binding and kinetic analysis, fhlA220 mRNA were transcribed using a DNA fragment that amplifies the FhlA gene from −220 to +60 in E. coli XL-10 cells using primers 5'-GGAACCGAATTCTAATACGACTCACTATAGCAGCGTTACATTCCCATCCACTGG-3' and 5'-CCGAGCGGATCCAATATTTGTCCGAGTGATGTCGAACAACCC-3' and digested with Ssp I before transcribing.

Therefore, we designed cleaved amplified polymorphic sequence (CAPS) markers that require the use of the restriction enzymes Nci I (BLZ1, SNP 1733), Ssp I (BLZ2, SNP 2161), and Sty I (BPBF, SNP -210).

Two approaches can be used to solve SSPs: (i) consider all probabilistically reachable states and (ii) plan only for a subset of these reachable states.

Managers reported employing an average of 6.9 (SD = 5.8, range = 0 30) Whole Time Equivalent (WTE) 'Specialist' SSPs (i.e. SSPs employed directly by the SSS specifically to deliver stop smoking interventions).

In addition, 84% of managers (n = 48) stated that their SSS was responsible for the support or performance management of 'Community' SSPs (i.e. SSPs, who deliver support for the SSS as part of or in addition to their main role, typically as practice nurses or community pharmacists), with 245 (SD = 335.0, range = 1 2000, median = 140) community SSPs being performance managed on average.

Characterization of the Z. tritici secretome yielded comparable results to the previously reported Z. tritici secretome (Morais Do Amaral et al. 2012), representing 874 secreted proteins including 441 small, secreted proteins (SSPs), i.e., with a size inferior to 300 amino acids (Table 1).

There was a total of 126 SSP MAP-positive herds (i.e., 21.3% SSP MAP-positive herds; 95% confidence interval = 18.0 24.9); the within-herd apparent prevalence (AP) ranged between 0.00 and 22.73% (mean 6.07%).