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Further investigation of SSC-specific miRNAs's functional annotation will allow insights into the mechanisms involved in the regulation of SSC activities.
Further investigation aimed at the functional dissection of these novel miRNAs could generate new insights into the regulation of SSC activities.
Meanwhile, several novel miRNAs were preferentially expressed in mouse SSCs, and further investigation of their functional annotation will allow insights into the mechanisms involved in the regulation of SSC activities.
These relationships may be explained by high-force, eccentric work when performing SSC activities such as high-speed running This force may exceed the muscles ability to actively resist load, forcing the muscle to lengthen and generate greater active tension [ 24].
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We found that GS cells show a constant level of SSC activity regardless of Kit expression.
Both Kit− and Kit+ cells in culture showed comparable levels of SSC activity after germ cell transplantation.
Second, immediately after transplantation, we found weaker but distinct SSC activity in the Kit+ donor cell population.
These results indicated that Kit+GS cells not only had SSC activity but also underwent self-renewal division at a level comparable to Kit− GS cells.
Another study also showed that Kit+ differentiating spermatogonia in the "side population", defined by the higher efflux of DNA-binding dye Hoeschst 33342, have SSC activity [32].
Although both phenotypic and functional analyses suggested that most GS cells are progenitors without SSC activity, single-cell cloning experiments in our previous study showed that a significant proportion of GS cells maintain a potential to function as SSCs [16].
After selection, 5.2±1.8% (n = 3) of the cultured cells could be recovered, and cells were then microinjected into seminiferous tubules of W mice. Two months after transplantation, analysis revealed that Kit-expressing cells have SSC activity.
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