Sentence examples for ssc 1 from inspiring English sources

All incubations and washes were done at 60°C with a final wash in 0.5% SSC, 1% SDS.

On SSC 1 in L we identified another suggestive QTL.

Posthybridization treatment included a washing procedure with 2 × SSC (3 × 5 minutes, at 50°C), 1 × SSC (1 × 5 minutes, at 60°C), 0.1 × SSC (1 × 15 minutes, at 60°C) and 0.05 × SSC (1 × 15 minutes, at 60°C).

Among these, 7 had SSc, 1 had SLE, 1 MCTD and 1 RA.

Finally, the membrane was washed in 2 × SSC, 1% SDS for 10 min.

The common QTLs were located at SSC 1, 2, 3, 7 and 15.

The RNA was blotted with 20× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate) onto a positively charged nylon membrane (Boehringer GmbH, Mannheim, Germany).

Activation of adjacent fibroblasts by CD4+ T cells is thought to be critical to the pathological fibrosis in SSc [ 1, 4, 5].

All patients with a suspected infection were screened for systemic inflammatory response syndrome (SIRS) and new onset organ failure criteria according to the SSC [1].

Cells were washed twice for 20 min. in 2× SSC/ 15% formamide and for 10 min. in SSC 1×.

Blots were washed three times in 2× SSC (1×SSC = 150 mM NaCl, 15 mM Na citrate, pH, 7.0), 1% SDS at room temperature, then two times for 30 min in 2×SSC, 1% SDS at 50°C, and finally one time for 30 min in 0.5×SSC, 1% SDS at 60°C.