Your English writing platform
Discover LudwigExact(4)
Comparison of torpedo-stage embryo expression profiles to QTL map data revealed candidate FHE genes, including the Arabidopsis Cyclophilin-40 homologue SQUINT (SQN), and functional studies revealed a previously uncharacterised role for SQN in stem cell regulation.
Mk. III went into extensive testing at No. 25 Sqn in May 1940 and another troubling problem was found.
In a reciprocal experiment, to address whether lower levels of SQN in ZLL-independent accessions such as Col-0 may be a reason for the absence of stem cell defects, SQN was ectopically expressed from the strong embryonic pAtRPS5a promoter in the Col zll ago10-1 mutant.
In combination with a zll mutation, which allows AGO1 greater access to miR165/166, increased levels of SQN in Ler-0 enhance repression of AGO1 targets, such as the Class III HD-ZIPs, and lead to a high frequency of terminal stem cell differentiation.
Similar(56)
Consistent with a role in modifying stem cell development, decreased SQN activity in Ler via sqn-4 and amiRSQN partially rescued stem cell maintenance in the zll-1 background, while increased SQN expression in Col zll ago10-1 induced stem cell termination.
Our current model for FHE2 function is based upon a conserved increase in SQN mRNA levels in Ler-0 and Fe-1 compared to Col-0.
Based on the antagonistic roles of AGO1 and ZLL in stem cell regulation, we speculated that increased SQN expression in Ler-0 might enhance AGO1 activity.
Taken together, these results suggest that different SQN expression levels in the Ler and Col accessions determine differences in stem cell termination in the absence of ZLL function, and that SQN is a strong candidate for a gene underlying the FHE2 QTL.> -wrap-foot> n = total seedlings counted.
Although changes in SQN expression alone cannot account for the drastic differences between zll phenotypes in Col-0 and Ler-0, it is likely that SQN forms part of an important pathway that contributes to ZLL function and FHE activity during stem cell development.
SQN mRNA levels in the ZLL-independent Ts-1 and Van-0 lines were unchanged relative to Fe-1/Ler-0.
Reduced SQN expression levels in Col-0 correlate with transposon insertions adjoining the transcriptional start site, which may contribute to stem cell maintenance in other ZLL-independent accessions.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com