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His diagnostics: inject patient's sputum into the stomach of a live mouse, autopsy the mouse, prescribe penicillin.
After taking a quick breath, the participants blew out again, and then coughed up sputum into a sterile container.
Subjects were asked to rinse their mouth out carefully and try to expectorate sputum into a container every five minutes.
To contain the spread of TB, the Japanese Governor-General of Korea installed bowls at various locations throughout cities and villages and ordered residents to spit their sputum into these containers.
Always between sessions the inhalation procedure was interrupted and the subjects were asked to blow their nose, rinse their mouth and try to expectorate sputum into a sterilized box.
Every 5 min, after the start of nebulisation, participants were asked to rinse their mouth and throat carefully, to discard saliva, and to try to cough sputum into a container; FEV1 was then measured.
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When an infectious person coughs, sneezes, spits, or even shouts, he sends minute particles of sputum, or phlegm, into the air — exposing anyone nearby.
After each dose, subjects were instructed to expectorate any sputum present into a Petri dish.
An exception to this was found for Price et al, who took morning sputum production into account, but with no timing reported for other symptoms.
Hypertonic sterile saline (5%) was nebulized for 30 minutes with an ultrasonic nebulizer (Fisoneb; Karapharm, Marseille, France); subjects rinsed their mouth with water every 10 minutes and tried to cough sputum directly into a sterile plastic box.
MMP-12 protein from R&D Systems was autocatalyzed and spiked into four human sputum samples and into assay diluent at three different concentrations (final theoretical spiking concentrations of 10,000, 3000, and 1000 pg/mL).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com