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Most of the spurious reads were short (average: 40 bases) and low-complexity (i.e. rich in nucleotide repeats).
Note that deletions are predicted even without missing coverage evidence in this case because it is possible for one or a few spurious reads or read alignments to prevent the prediction of a very small missing coverage evidence item.
Simply quantifying each individual restriction site as having reads aligning to it or not would fail to distinguish between restriction sites that would reliably generate sequencing reads and restriction sites that generated spurious reads from singular events.
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Spurious read mapping within the deletion, ambiguous split read alignments, gene translocations or heterozygous deletions could explain long-range deletions not meeting the aforementioned criteria.
We found no spurious HPV reads when the SiHa whole-genome sequence was reversed, suggesting the HPVDetector is specific to detect true HPV traces.
To estimate the rate of spurious alignments, reads were aligned against the genome of the modern chicken (Gallus gallus) using the May 2006, galGal3 assembly available at UCSC (http://hgdownload.cse.ucsc.edu/downloads.html chicken).html chicken
Some protocols use a PCR step, which may lead to the spurious replication of reads.
Note that some incompletely excised IESs may be spurious because of reads arising from contaminating micronuclear DNA.
Repetitive element assemblies are represented by all genomic instances (assembled from the RepeatMasker annotation) of an individual repetitive element subfamily, including flanking genomic sequences, concatenated with spacer sequences to avoid spurious mapping of reads spanning multiple instances.
Masked genomes help reduce spurious mapping when the reads contain sequencing errors.
A closer examination of the read mappings for both the PGM and MiSeq platforms indicated that some mapped reads were spurious.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com