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This step was important because one category of spurious alignment is the case where a pseudogene has been aligned in one or more lineages.
The short length of these fragments makes them more prone to spurious alignment and precludes accurate estimation of evolutionary distances.
All GeneSeqer alignments were evaluated by eye to verify acceptable exon sequence identity (i.e., no obvious paralogy or spurious alignment) between H. sanguinea and H. parviflora var.
All of the aforementioned errors can generate spurious alignment of non-homologous codons and significantly interfere with the reliable detection of positive selection [ 18, 19].
Only the two most significant alignments were retained in an attempt to avoid spurious alignment to multiple genes from different stickleback linkage groups (for example due to similarity of genes from the same gene family).
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To estimate the rate of spurious alignments, reads were aligned against the genome of the modern chicken (Gallus gallus) using the May 2006, galGal3 assembly available at UCSC (http://hgdownload.cse.ucsc.edu/downloads.html chicken).html chicken
More permissive alignment algorithms may also reduce the proportion of fragments that cannot be aligned, but could increase spurious alignments in complex genomes such as maize.
We found that spurious alignments often involved cases where the aligning fragment in one of the species did not come from a syntenic genomic region relative to the aligning fragments in other species.
The reference sequence for each genome must be considerably closer to that genome than to any of the others, but it is not necessary for the references to separate the sequences perfectly as the assembly graph can be used to identify spurious alignments, as well as to reallocate contigs that fail to align to any of the references.
On the other hand, short reads are more likely to cause spurious alignments.
In other words, the best alignment scores for spurious alignments depend on properties of the query map itself.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com