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The detailed force profiles of struts in the OT structure were further assessed during spreading (Supplementary Fig. S4).
This idea is supported by our observation that conventional (cPKC) and novel (nPKC) PKC isoforms are also not required for EPAC1-dependent cell spreading (Supplementary Fig. 6).
The intensities of FHOD1 and Myosin 1E were found to change in unison during the protrusive and contractile phases of lamellipodia spreading (supplementary material Movie 4).
Subsequent to the establishment of integrin KD in hMSC, we investigated for changes in their cell attachment, spreading (Supplementary Figures 2 and 4b) and migration on collagen I.
Tip accumulation of Myosin 1E increased as the lamellipodia moved forward in the contractile spreading phase and peaked at the end of active spreading (supplementary material Movie 1; Fig. 1H,I white arrows).
Treatment with channel blocking drugs, probenecid (Pbn, 1 mM, blocks pannexin channels) and lanthanum (La3+, 200 µM, blocks Cx channels) also had no effect on B cell spreading (supplementary material Fig. S2A D).
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Myosin 1EΔTH3 and TH1+TH2+TH3 were also found at actin rich early adhesion spots behind the tip of spreading lamellipodia (supplementary material Fig. S6A,B).
Two subjects given 0.6 units · kg−1 presented with M1 values more than three times the interquartile spread (Supplementary Fig. 1) that did not correlate with glucodynamic efficacy.
In addition, the X and Y chromosomes were partially synapsed at the pseudoautosomal region in both Bscl2+/+ and Bscl2−/− pachytene spreads (Supplementary Figure S5), indicating that Bscl2−/− spermatocytes did not present obvious synaptic errors during prophase I.
The variants appear spread within supplementary material and in tables.
While addition of the drug constricted the lamellipodia of RPTP cells and reduced cell area, washout restored normal surface area and lamellipodia spreading (Fig. 7I,J; supplementary material Movie 16).
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