We analyzed the degree of cytoskeleton spreading, expression of alkaline phosphatase, collagen type 1 and mineralization.
Unlike its effect on spreading, expression of T154A had minimal effects on the activation of Rap1 in response to BCR signaling (supplementary material Fig. S3C), even though these transfected cell lines had equivalent levels of membrane IgM (supplementary material Fig. S3D), ensuring equal stimulation through the BCR.
At 24 hpf, there was decreased expression in the floor plate while hypochord expression persisted with spreading ventral expression lateral to the dorsal aorta and posterior cardinal vein to the ventral somite boundary (Figure 2B and C).
(E ) Dorsal view of embryo as in (D ) with increased dorsal anterior expression of Nv hairy, and the anterior spreading of expression from the anterior of double-segment pair-rule stripe 1. (F ) Blastoderm embryo with expanding anterior domain (line), five double-segment 'pair-rule' stripes, and two additional stripes coming up.
Moreover, a distinct inhibition of the spreading and expression of brevican was observed surrounding the core of suppressed transduced U251 cells using a cytoimmunofluorescence assay.
It is expressed in many different human and mouse cell lines [16] suggesting wide-spread expression in vivo.
The spread expression of phosphorylated Rb in most suprabasal nuclei further suggests that they do not return to G1.
We tested the quality of the cDNA using a PCR for glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a housekeeping gene that has constitutive and wide-spread expression [45], [46].
The rapid rate of lactate assimilation, distribution and oxidation could be predicted given its central role in linking glycolytic and oxidative metabolism, as well as the wide-spread expression of lactate-hydrogen ion symporters (referred as monocarboxylate transporters, or MCTs) in muscle and other tissues [19], [22], [23], and the presence of a sodium ion-mediated intestinal MCT [24], [25].
Despite the wide-spread expression of the gene, the impact of Siglecg deletion appear to be limited to the B1a B cells in the peritoneal cavity, although a smaller expansion of the B1a B cells in the spleen can be observed at 2 weeks of age.
Our gene expression analysis revealed a wide spread expression of the reporter construct for sft-4 (see Table 2).
