On the other hand, the colonies on a cell culture polystyrene dish and a collagen-coated polystyrene dish showed indistinct outline and spread well, and most spreading cells only weakly expressed early undifferentiation markers.
Cell adhesion images, using immunofluorescent staining, of HNEpC on the Ti specimens (M, E1, and E2) after 12 h of cell incubation: dual staining of DAPI for nuclei (blue color) and rhodamine phalloidin for actin filaments (red color) (arrow: spreading cells).
Crystal violet staining micrographs of HNEpC on the Ti specimens (M, E1, and E2) after 12 h of cell incubation (a, b, and c: M, E1, and E2 at low magnification, respectively; d, e, and f: M, E1, and E2 at high magnification, respectively) (arrow: spreading cells).
First, after depolymerization of microtubules, spreading cells exhibited morphological oscillations.
No such organized actin network is observed in the spreading cells depleted of myosin IIA.
Scapinin was colocalized with actin at the edge of spreading cells.
Then we apply hypotheses generation to the problem of cortical oscillations of spreading cells [15], [24].
For western blot analysis, total lysates were made from the spreading cells.
Images of the spreading cells were collected using inverted light microscope.
As shown in the movie S1, GFP-Sec13 gives a largely diffused, cytosolic signal when overexpressed in actively spreading cells.
Anisotropic spreading cells do not, however, exhibit distinct phases of homogenous motility [30], more closely resembling filopodial dominated migration.
