Sentence examples for spread preparation from inspiring English sources
Normal spreads included typical metaphase spread preparation artifacts.
Colcemide (6 μg/mL) was added 45 minutes prior to harvesting cells for metaphase spread preparation.
Chromosome spread preparation of ovaries, in which soluble components not attached to chromatin are removed (Khetani and Bickel 2007), enabled us to determine whether Corolla localizes to meiotic chromosomes in the absence of c(3 G.
Third, Zhang et al. (2010b) note a high monosomy 7 incidence in the controls and indicate that this could be due to artifactual chromosome loss during meta-phase spread preparation; therefore, there is inherent bias in the sampling technique that could bias the results.
We used trochophore larvae at the early stage for chromosome spread preparation, and yet, no signal was observed on the maternal rRNA loci using paternal sequence as the probe in the FISH experiment, which suggests unequal crossover did not occur between paternal and maternal rRNA loci-containing chromosomes.
Pulsatile treatments of cultured MCF-7 cells with BMP7 (30 ng/mL) for 15 h thee times a week over two weeks resulted in significantly shortened telomeres (Fig. 1C and 1D), as revealed by quantitative fluorescence in situ hybridization (Q-FISH) using a specific telomeric DNA probe in the metaphase cell spread preparations.
Spread preparations of pachytene oocytes were obtained following the protocol in [44] for pachytene mapping.
Immunofluorescence techniques have been recently developed and widely used to determine genome-wide patterns of recombination on spread preparations of spermatocytes or oocytes from humans and animals [12] [15].
In nucleus spread preparations nuclear morphology is not original anymore.
To identify the W chromosome we examined spread preparations of pachytene oocytes using a combination of GISH and tel-FISH.
Micrographs of interphase nuclei were taken from DAPI-stained spread preparations of wing discs from three different larvae of each Leptidea species, using the same resolution.
