The map geometric characteristics include a limited number (∼4) of statistically significant dimensions, a bimodal distribution of the first component, increasing kurtosis of subsequent (unimodal) components, and a U-shaped maximum-spread planar projection.
These observations may go some way to explain the requirement for C/EBPδ for proper adhesion and spreading on planar and nanometric growth surfaces described in Fig. 1.
From these experiments it was apparent that MEF WT cells spread normally on planar growth surfaces, possessing a well developed actin cytoskeleton and adhesion complexes, as indicated by phalloidin and vinculin staining respectively.
The most stable core is non-planar, spread on both (11¯1) and the (111) planes, while the metastable one is planar, spread only on the (111) APB plane.
If no planar spreading occurs the dominant dislocations are determined by their energy.
These results demonstrate that the transcription factor C/EBPδ plays an essential role in efficient adhesion and spreading to both planar and nanometric grooved surfaces.
The functionalized microtubes offer a cell culture environment where the cells can form adhesions to the substrate all around the cell body so that a transition from a planar and spread to a more 3D morphology occurs.
Indeed, the well-known spreading (or dispersion) rate anomaly refers to the fact that traditional models do reasonably well in predicting the spreading rate of a planar jet but perform unexpectedly poor for rounds jets.
Analysis of spreading demonstrated that cell planar area was significantly higher on smooth surfaces at 2, 6 and 24 h post seeding in comparison with nanogrooves and discontinuous nanogrooves (p<0.05) (fig. 3B).
However, examining the majority of gene identities from C/EBPδ-dependent CLUSTERs, it is not immediately clear which genes might be directly responsible for the effects of C/EBPδ on cell adhesion, migration and spreading that we observed on planar and nanometric growth surfaces (Fig. 1).
