Exact(5)
The value of CFU was calculated as following: CFU mL - 1 = Number of colony counted/Amount of solution spread on plate /Dilution factor Open image in new window.
The solutions (0.1 ml) were spread on plate count agar (PCA) for B. subtilis, S. aureus, and S. enteritidis and potato dextrose agar (PDA) for A. niger.
Table 3 Bacteria colony counts before and after co-composting of pineapple leaves Sample Number of colony CFU mL-1of sample First day mixed co-compost (10-7 dilution) 155 1.55 × 1010 Final matured co-compost (10-5 dilution) 284 2.84 × 108 Chicken manure slurry (10-4 dilution) 113 1.13 × 107 CFU mL-1 = (Number of colony counted/Amount of spread on plate, mL /Dilution factor.
9. Carefully tilt the plate until top agar is spread on plate and allow to solidify.
Spread on plate evenly.
Similar(55)
For the remaining cells, aliquots of 100 μl were spread on one plate for a total of 10 plates and grown at 37°C for overnight.
Conidia (1 × 106) of mrTA495 were spread on plates containing 5-FOA, uridine, and arginine, and 120 colonies developed.
The suspended cells were spread on plates containing SD/-His/-Leu/-Trp medindicatedied with indiconcentrationratiof of 3-AT (3-amino-1, 2, 4-triazole) and ABA.
After transformation, the reactions were spread on plates containing ampicillin and X-Gal and the numbers of white and blue colonies were counted.
For HR and NHEJ assays, cells were grown in pre-induction medium (raffinose medium) and then spread on plates containing either galactose or glucose.
To evaluate the impact of pykA expression on the Ts character of the dnaE2.6 mutation, fresh dnaE2.6 Pspac-I::pykA cells grown at 30°C were spread on plates containing various IPTG concentrations and grown at restrictive temperature.
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