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The bacterial suspensions were evenly spread on agar media planted in Petri dishes.
In early attempts to isolate palm oil-utilising bacteria from palm oil mill effluent (POME), diluted liquid samples of POME were spread on agar containing POME as primary nutrient.
From each dilution, 0.1 ml was taken and spread on agar plates of medium containing in g/l oat spelt xylan 10, yeast extract 2, NaCl 2.5, NH4Cl 5, KH2PO4 15, Na2HPO4 30, MgSO4·7H2O 0.25, and bacteriological agar 15.
The cells were incubated for 60 min at 30°C, then washed with buffer and cell suspension was spread on agar plates containing 0.05 g/l aniline Blue after appropriate dilution.
The recovered cells were spread on agar plate of mineral salt (MS) medium (9 g of Na2HPO4·12H2O, 1.5 g of KH2PO4, 0.5 g of NH4Cl, 0.2 g of MgSO4·7H2O and 1 mL of trace element solution per liter of distilled water) (Kato et al. 1996) containing 1.5 g/L 4-aza-DL-leucine dihydrochloride (Sigma Aldrich, St Louis, MO, USA, Figure 1c) as a leucine analog.
Samples of the icing were spread on agar in petri dishes and the researchers waited 48 hours before discovering the gross truth about what's in our mouths.
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Third, the subsequent post-treatment of the sample after plasma treatment including the resuspension liquid and time, the subsequent dilution, and the volume used for spreading on agar.
All samples were spread on blood agar (blood agar base No.2; LabM, Salford, England + 5% horse blood) and incubated for 24 h at 37°C.
Ileo-caecal and rectal samples were spread on blood agar plates (blood agar base No.2; LabM, Salford, UK +5% horse blood) within 4 h after collection.
Faecal samples were spread on blood agar plates (blood agar base No. 2; LabM, Salford, England + 5% horse blood) and incubated for 18 h at 37°C.
The preculture was spread on the agar medium, and then the agar surface was overlaid with cyclohexane.
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