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Even an unregulated, constitutively expressed gene exhibits a 30% spread of expression levels over a population of identical cells [ 30].
Comparing these data from real cells with our simulated microarray data found that the datasets were notably different in the spread of expression ratios (Fig. 6).
This spread of expression values is consistent with that demonstrated within our own patient cohort, where some tumour samples show high level coordinate upregulation of FOXM1 and its targets whereas there are other tumours where the expression is generally lower.
Normalisation within a subgroup, like N, with an optimised reference gene combination (Supplementary Figures 1A and B for NT samples) results in reduced statistical spread of expression of inhibin genes, rather than in shift of the median expression, which varied between 0.86 and 1.22 only after normalisation for N samples (Supplementary Figure 1C).
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(E ) Dorsal view of embryo as in (D ) with increased dorsal anterior expression of Nv hairy, and the anterior spreading of expression from the anterior of double-segment pair-rule stripe 1. (F ) Blastoderm embryo with expanding anterior domain (line), five double-segment 'pair-rule' stripes, and two additional stripes coming up.
Due to the large number of patients and spread of gene expression values we quartiled each gene, giving us the following subgroups- 1 (low expression), 2 (low-medium), 3 (medium-high) and 4 (high).
Mutations in whorl 1 and 2 expressed AP1 or AP2 genes leads to a spread of AG expression from whorl 3 and 4 and results in carpels in whorl 1 and stamen in whorl 2. Additional mutations in whorl 2 and 3 expressed AP3 or PI genes show a further conversion to carpeloid organs [30].
These will differ in their cell-type-specific tropism, the efficiency of cellular transduction and the level and spread of gene expression mediated by the recombinant vectors.
Since the spread of mean expression values is larger in larger samples, we expect higher dispersion.
This spread of ATOH8 expression occurs progressively along the craniocaudal embryonic axis.
Table 1 presents the spread of gene expression in each of these bands and demonstrates that the majority of genes are expressed at the moderate to high level.
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