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Performing single-step and multi-step growth curves (starting MOI = 0.1 and 3 respectively) further revealed that TSA-enhanced VVdd growth was more substantial when the virus was provided at a low MOI and allowed to spread (Figure 1d).
To determine if these mutants behaved as motility mutants, they were tested in soft agar to determine the amount of colony spread (Figure 1), and they were observed microscopically both in wet mounts and after flagellar staining (Figure 2).
Focusing increases the energy spread (Figure 4b) and length (Figure 4c) of the pulse, besides distorting the pulse shape (Figure 4d).
The ΔΔGs for Tm-matched probe-pairs were also close to zero with little spread (Figure 3C).
In the subsubtype A3 analysis, we identified one monophyletic cluster (1979), suggesting a major introduction followed by local epidemic spread (Figure 1b).
Finally, there was a marked impact of temperature with larger outbreaks (Figure 1) and more extensive spread (Figure 2) for the 2006 compared with the 2007 temperatures.
Similar(47)
One or two additional, small, telocentric chromosomes are infrequently found in the metaphase spreads (Figure S2).
Approximately, 50% of the AtCRT1a and CRT3 expressing cells showed cell spreading, whereas wild-type cells typically had above 75% cell spreading (Figure 6A).
Similar morphological changes were observed in other epithelial cells where they appeared to be associated with enhanced cell spreading (Figure S1).
Deletion of the entire SCCmec type-III region from two of four strains, 86/961 and 85/3566, increased colony spreading (Figure S2), suggesting that fudoh in type-III SCCmec also has the function to suppress the colony spreading.
The early-passage (P2) MEFs which were grown in the presence of 10% fetal calf serum (FCS), were found to exhibit the same morphology (Figure 1B, left panels), spreading (Figure 1B, right panels) and plating efficiency (Figure 1C).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com