Oil-spreading analyses indicated that areas of oil-spreading zone in supernatant of ZS1 cultures containing 0.5, 1, 2 and 4% glucose supplements were 25, 50, 170, and 250 cm2/μl, respectively.
For cell spreading analyses, transduced cells were starved over night and seeded (3/1.5·10; Sum102/MDA-MB-231) on glass cover slips coated with fibronectin (10 μg/mL), laminin (10 μg/mL) or collagen I (10 μg/mL), and incubated for 1 hour at 37°C.
In agreement with a correlation between telomere processing and HOT1 telomere association, HOT1 robustly localized to telomeres on all spreads analysed with on average 87% of chromosome ends being HOT1 positive.
Their rotation paths and intragranular orientation spread were analysed in detail, using crystal plasticity modelling to evaluate the nature of the orientation spread.
We previously studied intrafamilial spread by analysing polymorphism of two housekeeping genes (hspA and glmM) following the failure of treatment to eradicate infection of a child [22].
To study the progress of infection during plant development, the time course of systemic spread was analysed for both ecotypes.
Here, we examine their origins and spread and analyse a large sample of native and invasive accessions with chloroplast and nuclear microsatellites.
G-banding was performed by the standard Giemsa staining procedure and metaphase spreads were analysed.
In total, 123 SPREADs were analysed from four independent experiments.
A total of 652 metaphase spreads were analysed in two specimens, 374 from cultures treated with 0.2 μM APC, and 278 from control cultures (table 1).
